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BAK1(BCL2-antagonist/killer 1) Antibody

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Catalog#   Accession # Size Price Qty
AVARP02022_P050   NP_001179 50µg $289.00
Description: 
BAK1 belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress.The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Gene Symbol: 
BAK1  
Alias Symbols: 
BAK; BAK-LIKE; BCL2L7; CDN1; MGC117255; MGC3887  
Peptide ID: 
P01109  
ELISA titre using peptide based assay: 
1:62500  
Search Keyword: 
BAK1, BAK; BAK-LIKE; BCL2L7; CDN1; MGC117255; MGC3887, NP_001179  
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Species Reactivity: 
(Primary - WB; Secondary - Computational Homology)
Human 
Antigen Specificity: 
Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human BAK1 with an internal ID of P01109 
Reconstitution and Storage: 
Antibody is lyophilized from PBS buffer with 2% sucrose. Add 50 μl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles. 
Key Reference: 
Dewson,G., (2008) Mol. Cell 30 (3), 369-380 
Quality Control: 
Rabbit Ig G is purified by peptide affinity chromatography method. 
Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. For research use only not for diagnostic, human, or veterinary use.
Western Blot
Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody.
Immunoblot

AVARP02022-QC0235-WB.jpg
 
Protein Accession# Swissprot Id Nucleotide Accession# Protein Size Molecular Weight
NP_001179 Q16611 NM_001188 211 23kDa