CCND2 Antibody (Phospho-Thr280) (OASG01936)

Catalog No: OASG01936

Size:100 uL

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: LiquidPBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: WB, IHC, ELISA
• Additional Information: Cellular Localization: Nucleus Cytoplasm Membrane. Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated into the nucleus through interaction with KIP/CIP family members. Isoform 2: Cytoplasm.
• Reconstitution and Storage: Store at -20C. Avoid repeated freeze/thaw cycles.
• Immunogen: Synthesized peptide derived from human Cyclin D2 around the phosphorylation site of T280. Location: 220-300aa.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Concentration: 1 mg/ml
• Target Post-Translational Modification: Phospho-Thr280
• Specificity: Phospho-Cyclin D2 (T280) Polyclonal Antibody detects endogenous levels of Cyclin D2 protein only when phosphorylated at T280.
• Application Info: WB: 1:500~2000
  IHC: 1:100~300
  ELISA: 1:10000

Target Info

• Gene Symbol: CCND2
• Gene Full Name: cyclin D2
• Alias Symbols: MPPH3, KIAK0002
• NCBI Gene Id: 894; 12444; 64033
• Description of Target: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK4 or CDK6 and functions as a regulatory subunit of the complex, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. Knockout studies of the homologous gene in mouse suggest the essential roles of this gene in ovarian granulosa and germ cell proliferation. High level expression of this gene was observed in ovarian and testicular tumors. Mutations in this gene are associated with megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 3 (MPPH3).
• Uniprot ID: P30279, P30280, Q04827
• Molecular Weight: 40 kDa