CCND1 Antibody (OAAD00148)

Catalog No: OAAD00148

Size:100 ug

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Clone: CY-D1
• Isotype: IgG2a
• Host: Mouse
• Application: WB, IHC, IP, ICC
• Reconstitution and Storage: At -20C for one year. After reconstitution, at 4C for one month. It can also be aliquotted and stored frozen at -20C for longer time.
• Immunogen: Recombinant human cyclin D1 protein.
• Purification: Purified by the goat anti-mouse IgG Affinity chromatography.
• Specificity: Human, mouse, rat.
  No cross reactivity with other proteins.
• Formulation: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.01mg NaN3 as preservative.
• Application Info: Western blot At 4ug/ml with the appropriate system to detect cyclin D1 in cells and tissues.
  Immunohistochemistry(P) At 8ug/ml to detect cyclin D1 in formalin fixed and paraffin embedded tissues.
  Immunohistochemistry(F) At 8ug/ml to detect cyclin D1 in formalin or acetone fixed tissues.
  Immunocytochemistry Suitable Other applications have not been tested.
  Optimal dilutions should be determined by end user.
• Storage Buffer: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml.
• Reference: 1. Rosenberg, C. L.; Kim, H. G.; Shows, T. B.; Kronenberg, H. M.; Arnold, A. : Rearrangement and overexpression of D11S287E, a candidate oncogene on chromosome 11q13 in benign parathyroid tumors. Oncogene 6: 449-453, 1991.
  2. Muller, H.; Lukas, J.; Schneider, A.; Warthoe, P.; Bartek, J.; Eilers, M.; Strauss, M. : Cyclin D1 expression is regulated by the retinoblastoma protein. Proc. Nat. Acad. Sci. 91: 2945-2949, 1994.
  3. Hinds, P. W.; Dowdy, S. F.; Eaton, E. N.; Arnold, A.; Weinberg, R. A. : Function of a human cyclin gene as an oncogene. Proc. Nat. Acad. Sci. 91: 709-713, 1994.

Target Info

• Gene Symbol: CCND1
• Gene Full Name: G1/S-specific cyclin-D1
• Alias Symbols: BCL1, PRAD1, U21B31, D11S287E
• NCBI Gene Id: 595
• Protein Name: G1/S-specific cyclin-D1
• Description of Target: D-type cyclins (cyclins D1, D2, and D3) are regarded as essential links between cell environment and the core cell cycle machinery. CyclinD1(CCND1), also known as BCL1. Expression of cyclin D1, but not of cyclins A and E, was induced by transfection of the RB gene into RB-deficient tumor cells. CCND1 gene to 11q13. cyclin D1 gene can function as an oncogene. Cyclin D1 expression is regulated by the retinoblastoma protein.
• Uniprot ID: P24385
• Protein Accession #: NP_444284.1
• Nucleotide Accession #: NM_053056.2