Catalog No: OKCA01670
Size:96 Wells
Price: $754.00
SKU
OKCA01670
Availability: Domestic: Antibody & Kits: 2 weeks | Proteins: 4-6 weeks | International: Antibody & Kits: 2 weeks | Proteins: 4-6 weeks
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Mouse | ||||||||||||||||||||||
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ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3.5 Hours | ||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | Aviva Systems Biology Ccnb1 ELISA Kit (Mouse) (OKCA01670) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ccnb1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ccnb1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Ccnb1 captured in the well. | ||||||||||||||||||||||
ELISA Kit Range | 15.6 - 1,000 pg/mL | ||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue Homogenates, Cell Lysates | ||||||||||||||||||||||
Sensitivity | 3.9 pg/mL | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sanadwich ELISA | ||||||||||||||||||||||
Protocol Information |
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Gene Symbol | CCNB1 |
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Gene Full Name | cyclin B1 |
Alias Symbols | CycB1, Cycb-4, Cycb-5, Ccnb1-r, Ccnb1-rs1, Cycb1-rs1, Ccnb1-rs13 |
NCBI Gene Id | 268697 |
Protein Name | G2/mitotic-specific cyclin-B1 |
Description of Target | Essential for the control of the cell cycle at the G2/M (mitosis) transition. |
Uniprot ID | P24860 |
Protein Accession # | NP_758505.2 |
Nucleotide Accession # | NM_172301.3 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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