- Gene Symbol:
- NCBI Gene Id:
- Official Gene Full Name:
- Cyclin A2
- Protein Accession #:
- Nucleotide Accession #:
- Alias Symbols:
- MGC156527, Ccna2
- Replacement Item:
- This antibody may replace item sc-136253 from Santa Cruz Biotechnology.
- Description of Target:
- The function of Ccna2 remains unknown.
- Protein Size (# AA):
- Molecular Weight:
- Affinity Purified
- WB, IHC
- Tissue Tool:
- Find tissues and cell lines supported by DNA array analysis to express Ccna2.
- RNA Seq:
- Find tissues and cell lines supported by RNA-seq analysis to express Ccna2.
- The immunogen is a synthetic peptide corresponding to a region of Rat
- Species Reactivity:
- Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Yeast, Zebrafish
- Predicted Homology Based on Immunogen Sequence:
- Cow: 100%; Dog: 92%; Guinea Pig: 100%; Horse: 92%; Human: 100%; Mouse: 92%; Rabbit: 100%; Rat: 100%; Sheep: 100%; Yeast: 82%; Zebrafish: 77%
- Complete computational species homology data:
- Anti-Ccna2 (ARP30159_P050)
- Peptide Sequence:
- Synthetic peptide located within the following region: TGYTLESLKPCLMDLHQTYLKAPQHAQQSIREKYKHSKYHSVSLLNPPET
- Product Format:
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
- Reconstitution and Storage:
- For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
- Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
- Blocking Peptide:
- For anti-Ccna2 (ARP30159_P050) antibody is Catalog # AAP30159 (Previous Catalog # AAPS08605)
- Datasheets / Downloads:
- Printable datasheet for anti-Ccna2 (ARP30159_P050) antibody
University of Columbia Medical Center
Aviva Systems Biology is the original manufacturer of this Ccna2 antibody (ARP30159_P050)
Click here to view the Ccna2 antibody Western Blot Protocol
Product Datasheet Link: Ccna2 antibody (ARP30159_P050)
WB Suggested Anti-Ccna2 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Rat Muscle
Western Blot image:
Description of Target: The function of Ccna2 remains unknown.
Questions pertaining to this data can be directed to firstname.lastname@example.org
Aviva Systems Biology’s Ccna2 antibody (ARP30159_P050) has been tested using other cell lysates and tissues. To obtain more data about this antibody please email us at email@example.com.
To order by phone call us at (888) 880-0001, fax us at (858) 552-6975 or send an email to firstname.lastname@example.org. Aviva manufactures this antibody so we can offer the best price. Please contact us to request pricing information.
All of Aviva’s products are guaranteed for the applications and experimental sample types mentioned in the datasheet below. Are you curious if this product will work for you? Please contact us at email@example.com
Product Protocol: CCNA2 Antibody (ARP30159_P050) in Human Lymph Node Tissue using Immunohistochemistry
Aviva Systems Biology is the original manufacturer of this CCNA2 antibody.
Product Datasheet Link: CCNA2 antibody ARP30159_P050
Rabbit Anti-CCNA2 Antibody
Catalog Number: ARP30159_P050
Formalin Fixed Paraffin Embedded Tissue: Human Lymph Node Tissue
Observed Staining: Cytoplasm
Primary Antibody Concentration: 1:600
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Exposure Time: 0.5 - 2.0 sec
Left to right:
DAPI, CCNA2 Ab, Merge
Control Antibody: Normal Rabbit IgG
Control Antibody Concentration: 1:100
Left to right:
DAPI, Rabbit IgG, Merge
1. Normal adult human lymph node tissue was formalin fixed, embedded in paraffin wax, sectioned at 6 micron thickness and put on histological slides.
2. After deparaffinization and rehydration, the low pH, heat-induced antigen retrieval method utilizing Sodium Citrate buffer was performed.
3. The blocking buffer was 5% normal goat serum.
4. Primary antibodies was diluted in antibody dilution buffer (1% Normal Donkey Serum) to the final testing dilution (1:100, 1:600, 1:1200).
5. The appropriate anti-rabbit fluorescent-conjugated (Rhodamine:red or FITC:green) secondary antibody was applied and nuclei will be counterstained with DAPI (blue).
6. A Negative control utilized a nonspecific rabbit IgG staining the same normal adult human lymph node tissue.