Catalog No: OKCD02720
Size:96WELLS
Price: $725.00
SKU
OKCD02720
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Datasheets/ManualsPrintable datasheet for CCL8 ELISA Kit (Pig) (OKCD02720)
Product Info
Predicted Species ReactivityPorcine|Sus scrofa
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)96-105%78-88%85-94%87-96%
EDTA Plasma (n=5)93-101%91-98%81-99%96-104%
Heparin Plasma (n=5)93-105%79-102%78-101%94-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Monocyte Chemotactic Protein 2 (MCP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Monocyte Chemotactic Protein 2 (MCP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Monocyte Chemotactic Protein 2 (MCP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Monocyte Chemotactic Protein 2 (MCP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range7.81-500pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)82-10295
EDTA Plasma (n=5)87-9693
Heparin Plasma(n=5)85-9993
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Monocyte Chemotactic Protein 2 (MCP2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Monocyte Chemotactic Protein 2 (MCP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CCL8 Microplate96 Wells (12 x 8 Well strips)
CCL8 Lyophilized Standard2
100X Biotinylated CCL8 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity3.3 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Monocyte Chemotactic Protein 2 (MCP2). No significant cross-reactivity or interference between Monocyte Chemotactic Protein 2 (MCP2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolCCL8
Alias SymbolsMonocyte chemoattractant protein 2;Monocyte chemotactic protein 2;Small-inducible cytokine A8.
Protein NameC-C motif chemokine 8
Description of TargetChemotactic factor that attracts monocytes. This protein can bind heparin.
Uniprot IDP49873
Protein Accession #NP_001157987.1
Nucleotide Accession #NM_001164515.1
Protein Size (# AA)99
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