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Catalog No: OKBB00388
Size:96 Wells
Price: $583.00
SKU
OKBB00388
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva's rat CCL7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL7 has been precoated onto 96-well plates. Standards(E.coli, Q24-P97) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the rat CCL7 amount of sample captured in plate.
ELISA Kit Range15.6 pg/ml - 1,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n 161616242424
Mean (pg/mL)145382584160433627
SD6.9613.3722.198.6424.2531.98
CV%4.83.53.85.45.65.1
ELISA Kit Component
ComponentAmount
96-Well plate Pre-coated with Anti-Rat MCP-3 Antibody1 x (12 x 8 Well Strip)
Lyophilized Recombinant Rat MCP-3 Standard2 x 10 ng/tube
100X Biotinylated Anti-Rat MCP-3 Antibody1 x 130 uL
100X Avidin-Biotin-Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeCell culture supernatants, serum and plasma (heparin, EDTA)
Sensitivity<10 pg/ml
SpecificityNatural and recombinant rat CCL7
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
ImmunogenExpression system for standard: E.coli; Immunogen sequence: Q24-P97
DescriptionFor quantitative detection of rat CCL7 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Gene SymbolCCL7
Gene Full Namechemokine (C-C motif) ligand 7
Alias SymbolsC-C motif chemokine 7, Ccl7, CCL7_HUMAN, Chemokine CC Motif Ligand 7, Chemotactic protein 3, FIC, Intercrine/chemokine MARC, MARC, MCP 3, MCP-3, Monocyte chemoattractant protein 3, Monocyte chemotactic protein 3, NC28, RANTES, sis homolog, RP23-350G1.4, SCYA6, SCYA7, Small inducible cytokine A7, Small-inducible cytokine A7
NCBI Gene Id287561
Protein NameC-C motif chemokine 7
Description of TargetChemokine (C-C motif) ligand 7 (CCL7) is a small cytokine known as a chemokine that was previously called monocyte-specific chemokine 3 (MCP3). It belongs to the C-C chemokine family. By fluorescence in situ hybridization, mapped the MCP3 gene to chromosome 17q11.2-q12.
Uniprot IDQ9QXY8
Protein Accession #NP_001007613.1
Nucleotide Accession #NM_001007612.1
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