CCL4/MIP-1 beta ELISA Kit (Rat) (OKBB00467)

Catalog No: OKBB00467

Size:96 Wells

Rat CCL4/MIP-1 beta ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's rat CCL4 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL4 has been precoated onto 96-well plates. Standards(E.Coli, A24-N92) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the rat CCL4 amount of sample captured in plate.
• ELISA Kit Range: 15.6 pg/ml - 1,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	35	112	367	36	116	371
  SD	1.3	6.1	21.3	1.9	7.4	26.7
  CV%	3.7	5.4	5.8	5.3	6.4	7.2

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Rat MIP-1 beta Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Rat MIP-1 beta Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Rat MIP-1 beta Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant rat CCL4
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: A24-N92
• Description: For quantitative detection of rat CCL4 in cell culture supernatants, serum and plasma(heparin, EDTA, citrate).

Target Info

• Gene Symbol: CCL4
• Gene Full Name: chemokine (C-C motif) ligand 4
• Alias Symbols: ACT-2, ACT2, AT744.1, C-C motif chemokine 4, CC chemokine ligand 4, CCL4, CCL4_HUMAN, G-26 T-lymphocyte-secreted protein, HC21, LAG-1, LAG1, Lymphocyte activation gene 1 protein, Macrophage inflammatory protein 1-beta, MIP-1-beta, MIP-1-beta(1-69), MIP-1-beta(3-69), MIP1-B, MIP1B, MIP1B1, PAT 744, Protein H400, SCYA2, SCYA4, SIS-gamma, Small-inducible cytokine A4, T-cell activation protein 2
• NCBI Gene Id: 116637
• Protein Name: C-C motif chemokine 4
• Description of Target: Chemokine (C-C motif) ligand 4, also known as CCL4, is a protein which in mouse is encoded by the CCL4 gene. It is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Performing-low memory CD8+ T cells that normally synthesize MIP-1-beta. By analysis of somatic cell hybrids and by in situ hybridization, the CCL4 gene was assigned to 17q21-q23. Based on functional analysis of MIP-1β, one or more additional β chemokine receptors that transduce MIP-1β signals must also exist. MIP-1α and MIP-1β have been shown to lack affinity for the erythrocyte surface chemokine receptor/Duffy antigen that binds many α as well as β chemokines (11, 14).
• Uniprot ID: P50230
• Protein Accession #: NP_446310.1
• Nucleotide Accession #: NM_053858.1