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Catalog No: OKBB00419
Size:96 Wells
Price: $583.00
SKU
OKBB00419
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva's mouse CCL28 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CCL28 has been precoated onto 96-well plates. Standards(E.coli, I23-R130) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL28 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse CCL28 amount of sample captured in plate.
ELISA Kit Range31.2 pg/ml - 2,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n 161616242424
Mean (pg/mL)682721013712831154
SD4.8917.1438.496.3221.5183.09
CV%7.26.33.88.97.67.2
ELISA Kit Component
ComponentAmount
96-Well plate Pre-coated with Anti-Mouse CCL28 Antibody1 x (12 x 8 Well Strip)
Lyophilized Recombinant Mouse CCL28 Standard2 x 10 ng/tube
100X Biotinylated Anti-Mouse CCL28 Antibody1 x 130 uL
100X Avidin-Biotin-Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeCell culture supernatants, serum and plasma (heparin, EDTA)
Sensitivity<10 pg/ml
SpecificityNatural and recombinant mouse CCL28
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
ImmunogenExpression system for standard: E.coli; Immunogen sequence: I23-R130
DescriptionFor quantitative detection of mouse CCL28 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Gene SymbolCCL28
Gene Full Namechemokine (C-C motif) ligand 28
Alias SymbolsCCK 1, CCK1, CCL 28, Chemokine (C-C motif) ligand 28, MEC, MGC71902, Mucosae associated epithelial chemokine, OTTHUMP0000022162, SCYA28, Small inducible cytokine A28, Small inducible cytokine A28 [Precursor], Small inducible cytokine subfamily A (Cys-Cys), member 28
NCBI Gene Id56838
Protein NameC-C motif chemokine 28
Description of TargetCCL28, also known as mucosae-associated epithelial chemokine (MEC), CCK1 and SCYA28, is a chemokine. It is mapped to 5p12. The tumor hypoxia promotes the recruitment of regulatory T (Treg) cells through induction of expression of the chemokine CCL28, which in turn promotes tumor tolerance and angiogenesis. CCL28 regulates the chemotaxis of cells that express the chemokine receptors CCR3 and CCR10. CCL28 has also been implicated in the migration of IgA-expressing cells to the mammary gland, salivary gland, intestine and other mucosal tissues. It has also been shown as a potential antimicrobial agent effective against certain pathogens, such as Gram negative and Gram positive bacteria and the fungus Candida albicans.
Uniprot IDQ9JIL2
Protein Accession #NP_064675.1
Nucleotide Accession #NM_020279.3
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