CCL24/Eotaxin-2 ELISA Kit (Mouse) (OKBB00408)

Catalog No: OKBB00408

Size:96 Wells

Mouse CCL24/Eotaxin-2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's mouse CCL24 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CCL24 has been precoated onto 96-well plates. Standards(E.coli, V27-V119) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL24 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse CCL24 amount of sample captured in plate.
• ELISA Kit Range: 15.6 pg/ml - 1,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	125	257	526	130	249	518
  SD	6	10.79	33.14	10.14	15.94	42.99
  CV%	4.8	4.2	6.3	7.8	6.4	8.3

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Mouse Eotaxin-2 Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Mouse Eotaxin-2 Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Mouse Eotaxin-2 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum and plasma (heparin, EDTA)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant mouse CCL24
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: V27-V119
• Description: For quantitative detection of mouse CCL24 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: CCL24
• Gene Full Name: chemokine (C-C motif) ligand 24
• Alias Symbols: C C motif chemokine 24, CCL24, Chemokine (C C motif) ligand 24, Chemokine CC Motif Ligand 2, CK beta 6, Ckb 6, Ckb6, Eosinophil chemotactic protein 2, Eotaxin 2 like protein, MPIF 2, MPIF2, Myeloid progenitor inhibitory factor 2, OTTHUMP00000210225, SCYA24, Small inducible cytokine A24, Small inducible cytokine subfamily A (Cys Cys) member 24
• NCBI Gene Id: 56221
• Protein Name: C-C motif chemokine 24
• Description of Target: Chemokine (C-C motif) ligand 24 (CCL24) also known as myeloid progenitor inhibitory factor 2 (MPIF-2) or eosinophil chemotactic protein 2 (eotaxin-2) is a protein that in humans is encoded by the CCL24 gene.1 By use of PCR analysis of somatic cell hybrid DNAs, radiation hybrid mapping, and a chromosome 7-specific YAC library, mapped the SCYA24 gene to chromosome 7q11.23.2 CCL24 is a small cytokine belonging to the CC chemokine family. CCL24 interacts with chemokine receptor CCR3 to induce chemotaxis in eosinophils.3 This chemokine is also strongly chemotactic for resting T lymphocytes and slightly chemotactic for neutrophils.
• Uniprot ID: Q9JKC0
• Protein Accession #: NP_062523.1
• Nucleotide Accession #: NM_019577.4