CCL22 ELISA Kit (Mouse) (OKEH04046)

Catalog No: OKEH04046

Size:96T

Mouse CCL22 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Ccl22 ELISA Kit (Mouse) (OKEH04046) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ccl22 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ccl22 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ccl22 captured in the well.
• ELISA Kit Range: 15.6-1000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 7.5% (n = 20)
  Mean Inter-assay CV%: <= 9.9% (n = 20)
• ELISA Kit Component:
  • Anti-Ccl22 Microplate: 96 Wells (12 x 8 Well Strips)
  • Ccl22 Lyophilized Standard: 2 x 2 ng
  • Sample Diluent: 1 x 20 mL
  • Ccl22 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 7.87 pg/mL
• Specificity: Natural and recombinant Mouse C-C motif chemokine 22
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Ccl22
• Gene Full Name: chemokine (C-C motif) ligand 22
• Alias Symbols: ABCD-;ABCD-1;activated B and dendritic cell-derived;CC chemokine ABCD-1;C-C motif chemokine 22;DCBCK;dendritic cell and B cell derived chemokine;MDC;Scya;Scya22;SMALL INDUCIBLE CYTOKINE A22 PRECURSOR (CC CHEMOKINE ABCD-1) (ACTIVATED B AND DENDRITIC CELL-DERIVED);small inducible cytokine subfamily A, member 22;small inducible cytokine subfamily A22;small-inducible cytokine A22.
• NCBI Gene Id: 20299
• Protein Name: O88430
• Description of Target: C-C motif chemokine 22
• Uniprot ID: https://www.uniprot.org/uniprot/O88430
• Protein Accession #: NP_033163.1
• Nucleotide Accession #: NM_009137.2
• Protein Size (# AA): 92
• Molecular Weight: 10 kDa