CCL20 ELISA Kit (Mouse) (OKEH04047)

Catalog No: OKEH04047

Size:96T

Mouse CCL20 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Ccl20 ELISA Kit (Mouse) (OKEH04047) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ccl20 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ccl20 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ccl20 captured in the well.
• ELISA Kit Range: 3.9-250pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 6.5% (n = 20)
  Mean Inter-assay CV%: <= 8.2% (n = 20)
• ELISA Kit Component:
  • Anti-Ccl20 Microplate: 96 Wells (12 x 8 Well Strips)
  • Ccl20 Lyophilized Standard: 2 x 2 ng
  • Sample Diluent: 1 x 20 mL
  • Ccl20 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 1.97 pg/mL
• Specificity: Natural and recombinant Mouse C-C motif chemokine 20
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Ccl20
• Gene Full Name: chemokine (C-C motif) ligand 20
• Alias Symbols: beta-chemokine exodus-1;CC chemokine LARC;CC chemokine ST38;C-C motif chemokine 20;CKb4;exodus;exodus-1;LARC;liver and activation-regulated chemokine;macrophage inflammatory protein 3 alpha;MI;MIP;MIP-3;MIP-3[a];MIP3A;MIP-3A;MIP-3-alpha;Scya;Scya20;small inducible cytokine subfamily A20;small-inducible cytokine A20;ST3;ST38.
• NCBI Gene Id: 20297
• Protein Name: O89093
• Description of Target: C-C motif chemokine 20
• Uniprot ID: https://www.uniprot.org/uniprot/O89093
• Protein Accession #: NP_001153210.1
• Nucleotide Accession #: NM_001159738.1
• Protein Size (# AA): 97
• Molecular Weight: 11 kDa