CCL1 ELISA Kit (Human) (OKBB00406)

Catalog No: OKBB00406

Size:96 Wells

Human CCL1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's human CCL1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL1 has been precoated onto 96-well plates. Standards(NS0, K24-K96) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human CCL1 amount of sample captured in plate.
• ELISA Kit Range: 15.6 pg/ml - 1,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	46	125	738	48	127	741
  SD	2.1	6.4	53.1	3	8.5	54.8
  CV%	4.6	5.1	7.2	6.3	6.7	7.4

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Human CCL1 Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Human CCL1 Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Human CCL1 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants serum and plasma (heparin, EDTA)
• Sensitivity: <4 pg/ml
• Specificity: Natural and recombinant human CCL1
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: K24-K96
• Description: For quantitative detection of human CCL1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: CCL1
• Gene Full Name: chemokine (C-C motif) ligand 1
• Alias Symbols: BF534335, C C motif chemokine 1, C-C motif chemokine 1, Ccl1, CCL1_HUMAN, CCR8 ligand, Chemokine CC Motif Ligand 1, Inflammatory cytokine I-309, P500, RP23-446K18.2, SCYA1, SISe, Small inducible cytokine A1, Small-inducible cytokine A1, T lymphocyte secreted protein I-309, T lymphocyte-secreted protein I-309, TCA3
• NCBI Gene Id: 6346
• Protein Name: C-C motif chemokine 1
• Description of Target: CCL1, Chemokine (C-C motif) ligand 1, is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine is secreted by activated T cells and displays chemotactic activity for monocytes but not for neutrophils. It binds to the chemokine receptor CCR8.
• Uniprot ID: P22362
• Protein Accession #: NP_002972.1
• Nucleotide Accession #: NM_002981.2