CCKBR ELISA Kit (Human) (OKCD02432)

Catalog No: OKCD02432

Size:96WELLS

Human CCKBR ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cholecystokinin B Receptor (CCKBR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cholecystokinin B Receptor (CCKBR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cholecystokinin B Receptor (CCKBR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cholecystokinin B Receptor (CCKBR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cholecystokinin B Receptor (CCKBR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cholecystokinin B Receptor (CCKBR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CCKBR Microplate: 96 Wells (12 x 8 Well strips)
  • CCKBR Lyophilized Standard: 2
  • 100X Biotinylated CCKBR Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Tissue homogenates and other biological fluids
• Sensitivity: 0.052 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cholecystokinin B Receptor (CCKBR). No significant cross-reactivity or interference between Cholecystokinin B Receptor (CCKBR) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CCKBR
• Gene Full Name: cholecystokinin B receptor
• Alias Symbols: GASR, CCK-B, CCK2R
• NCBI Gene Id: 887
• Protein Name: Gastrin/cholecystokinin type B receptor
• Description of Target: Receptor for gastrin and cholecystokinin. The CKK-B receptors occur throughout the central nervous system where they modulate anxiety, analgesia, arousal, and neuroleptic activity. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Isoform 2 is constitutively activated and may regulate cancer cell proliferation via a gastrin-independent mechanism.
• Uniprot ID: P32239
• Protein Accession #: NP_001304958.1
• Nucleotide Accession #: NM_001318029.1
• Protein Size (# AA): 447