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Catalog No: OKCD02430
Size:96WELLS
Price: $625.00
SKU
OKCD02430
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Datasheets/ManualsPrintable datasheet for CCK ELISA Kit (Mouse) (OKCD02430)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration2h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)88-95%83-97%78-101%93-105%
EDTA Plasma (n=5)85-93%83-103%95-103%80-94%
Heparin Plasma (n=5)85-103%86-99%80-91%82-104%
ELISA Kit PrincipleThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Cholecystokinin (CCK) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Cholecystokinin (CCK) and unlabeled Cholecystokinin (CCK) (Standards or samples) with the pre-coated antibody specific to Cholecystokinin (CCK). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Cholecystokinin (CCK) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Cholecystokinin (CCK) in the sample.
ELISA Kit Range12.35-1,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)88-10192
EDTA Plasma (n=5)80-9086
Heparin Plasma (n=5)88-9893
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cholecystokinin (CCK) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cholecystokinin (CCK) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Cck Microplate96 Wells (12 x 8 Well strips)
Cck Lyophilized Standard 2 x 1 ng
100X Cck Biotin Complex1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 4.82 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cholecystokinin (CCK). No significant cross-reactivity or interference between Cholecystokinin (CCK) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Competitive Inhibition ELISA
Gene SymbolCck
Gene Full Namecholecystokinin
Alias Symbolscholecystokinin.
NCBI Gene Id12424
Protein NameCholecystokinin
Description of TargetThis peptide hormone induces gall bladder contraction and the release of pancreatic enzymes in the gut. Its function in the brain is not clear. Binding to CCK-A receptors stimulates amylase release from the pancreas, binding to CCK-B receptors stimulates gastric acid secretion.
Uniprot IDP09240
Protein Accession #NP_001271437.1
Nucleotide Accession #NM_001284508.2
Molecular Weight115 kDa
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