CBS Antibody - N-terminal region (ARP45746_T100)

Catalog No: ARP45746_T100

Host: Rabbit
Target Name: CBS
Sample Type: HepG2
Lane A: Primary Antibody
Lane B: Primary Antibody + Blocking Peptide
Primary Antibody Concentration: 1ug/ml
Peptide Concentration: 5ug/ml
Lysate Quantity: 25ug/lane/lane
Gel Concentration: 0.12

Host: Rabbit
Target Name: CBS
Sample Tissue: Rat Liver
Antibody Dilution: 1ug/ml

Host: Rat
Target Name: CBS
Sample Tissue: Rat Liver
Antibody Dilution: 1ug/ml

Kidney

Mouse Brain

Product Info

• Tested Species Reactivity: Human, Mouse, Rat
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Yeast, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: IHC, WB
• Additional Information: IHC Information: Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 1.25 ug/ml. Gel concentration: 12%.
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CBS
• Purification: Protein A purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Yeast: 86%; Zebrafish: 93%
• Peptide Sequence: Synthetic peptide located within the following region: RCIIVMPEKMSSEKVDVLRALGAEIVRTPTNARFDSPESHVGVAWRLKNE
• Concentration: 1.0 mg/ml
• Blocking Peptide: For anti-CBS (ARP45746_T100) antibody is Catalog # AAP45746 (Previous Catalog # AAPP26703)
• Enhanced Validation:

  WB	Y
  SPR
  YCHAROS

• Reference: Urreizti,R., (2006) J. Hum. Genet. 51 (4), 305-313
• Publications:

  Dahlhoff, C. et al. Hepatic methionine homeostasis is conserved in C57BL/6N mice on high-fat diet despite major changes in hepatic one-carbon metabolism. PLoS One 8, e57387 (2013). 23472083

  Dahlhoff, C. et al. Methyl-donor supplementation in obese mice prevents the progression of NAFLD, activates AMPK and decreases acyl-carnitine levels. Mol. Metab. 3, 565-80 (2014). 25061561

  Fernandes, V. S. et al. Endogenous hydrogen sulfide has a powerful role in inhibitory neurotransmission to the pig bladder neck. J. Urol. 189, 1567-73 (2013). 23063804

  Geillinger, K. E. et al. Hepatic metabolite profiles in mice with a suboptimal selenium status. J. Nutr. Biochem. 25, 914-22 (2014). 24917045

  Markand, S. et al. Cystathionine beta synthase expression in mouse retina. Curr. Eye Res. 38, 597-604 (2013). 23470016

  Porteus, C. S. et al. The role of hydrogen sulphide in the control of breathing in hypoxic zebrafish (Danio rerio). J. Physiol. 592, 3075-88 (2014). 24756639

  Spellman, C., Ahmed, M. M., Dubach, D. & Gardiner, K. J. Expression of trisomic proteins in Down syndrome model systems. Gene 512, 219-225 (2013). 23103828

  Yamane, S., Kanno, T., Nakamura, H., Fujino, H. & Murayama, T. Hydrogen sulfide-mediated regulation of contractility in the mouse ileum with electrical stimulation: Roles of l-cysteine, cystathionine beta-synthase, and K(+) channels. Eur. J. Pharmacol. 740, 112-20 (2014). 25008073

Target Info

• Gene Symbol: CBS
• Gene Full Name: Cystathionine-beta-synthase
• Alias Symbols: CBSL, HIP4
• NCBI Gene Id: 875
• Protein Name: Cystathionine beta-synthase
• Description of Target: CBS is involved in the transsulfuration pathway. The first step of this pathway, from homocysteine to cystathionine, is catalyzed by this protein. CBS deficiency can cause homocystinuria which affects many organs and tissues, including the eyes and the skeletal, vascular and central nervous systems.The protein encoded by this gene is involved in the transsulfuration pathway. The first step of this pathway, from homocysteine to cystathionine, is catalyzed by this protein. CBS deficiency can cause homocystinuria which affects many organs and tissues, including the eyes and the skeletal, vascular and central nervous systems.
• Uniprot ID: P35520
• Protein Accession #: NP_000062
• Nucleotide Accession #: NM_000071
• Protein Size (# AA): 551
• Molecular Weight: 61 kDa
• Protein Interactions: UBASH3A; UBE2I; PSMA1; PRKAG1; PIN1; EHHADH; CBS; SUMO1; UBC; NEDD8; PIAS3; RANBP9; PIAS1; CBX4; SRP14; RAD23B; PKM; NUCB1; IREB2; HSF1; LRSAM1; C14orf142; UBA5; IPO4; GOPC; PARP6; NPLOC4; DERA; ERO1L; DBNL; GPN1; PDLIM5; PLIN3; UBA2; GPSM1; COL4A3BP; UGP