CAT ELISA Kit (Mouse) : 96 Wells (OKEH00329)

Catalog No: OKEH00329 (Formerly GWB-MCIAMZ)

Size:96T

Mouse CAT ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology CAT ELISA Kit (Mouse) (OKEH00329) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-CAT antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated CAT and incubated. The CAT found in the sample or standards competes with the biotinylated CAT for limited binding sites on the immobilized anti- CAT antibody. Excess unbound biotinylated CAT and sample or standard CAT is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated CAT captured in the well and inversely proportional to the amount of CAT which was contained in the sample or standard.
• ELISA Kit Range: 15.6-1000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 103%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.5% (n = 20)
  Mean Inter-assay CV%: < 8.6% (n = 20)
• ELISA Kit Component:
  • CAT Microplate: 96 Wells (12 x 8 Well strips)
  • CAT Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated CAT Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 7.87 pg/mL
• Specificity: Natural and recombinant Mouse Catalase
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: Cat
• Gene Full Name: catalase
• Alias Symbols: 2210418N07;Ca;Cas;Cas1;Cas-1;catalase;catalase 1;Cs-;Cs-1.
• NCBI Gene Id: 12359
• Protein Name: P24270
• Description of Target: Catalase
• Uniprot ID: https://www.uniprot.org/uniprot/P24270
• Protein Accession #: NP_033934.2
• Nucleotide Accession #: NM_009804.2
• Protein Size (# AA): 527
• Molecular Weight: 60 kDa