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Datasheets/Manuals | Printable datasheet for CASP9 Antibody (OAPB00050) (OAPB00050) |
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Predicted Species Reactivity | Human|Mouse|Rat |
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Product Format | Liquid. Caspase-9 Antibody is supplied in PBS containing 0.02% sodium azide. |
Clonality | Polyclonal |
Host | Rabbit |
Conjugation | Unconjugated |
Application | Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunoprecipitation|Western blot |
Additional Information | HeLa Cell Lysate (OCPB00181) |
:: | Buffer: Antibody is supplied in PBS containing 0.02% sodium azide. |
Reconstitution and Storage | Caspase-9 antibody can be stored at 4C for three months and-20C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Immunogen | Anti-Caspase 9 antibody (2073) was raised against a peptide corresponding to 20 amino acids near the center of human Caspase 9. The immunogen is located within amino acids 290-340 of Caspase 9. |
Purification | Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Human |
Concentration | 1 mg/ml |
Blocking Peptide | For anti-CASP9 (OAPB00050) antibody is Catalog # OEPB02178 |
Specificity | It has no cross response to other members in caspase family. |
Application Info | Caspase-9 antibody can be used for detection of caspase-9 by Western blot at 1:1000 dilution. A 46 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 2 ug/mL. For immunofluorescence start at 20 ug/mL. Optimal dilutions for each application to be determined by the researcher. |
Reference | Duan H, Orth K, Chinnaiyan AM, Poirier GG, Froelich CJ, He WW, Dixit VM. ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B. J Biol Chem 1996;271:16720-4; Srinivasula SM, Fernandes-Alnemri T, Zangrilli J, Robertson N, Armstrong RC, Wang L, Trapani JA, Tomaselli KJ, Litwack G, Alnemri ES. The Ced-3/interleukin 1β converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2α are substrates for the apoptotic mediator CPP32. J Biol Chem 1996;271:27099-106 |
Gene Symbol | CASP9 |
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Gene Full Name | caspase 9 |
Alias Symbols | APAF3;APAF-3;apoptotic protease activating factor 3;apoptotic protease MCH-6;Apoptotic protease-activating factor 3;caspase 9, apoptosis-related cysteine peptidase;caspase-9;ICE-LAP6;ICE-like apoptotic protease 6;MCH6;PPP1R56;protein phosphatase 1, regulatory subunit 56. |
NCBI Gene Id | 842 |
Protein Name | Caspase-9 |
Description of Target | Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). |
Uniprot ID | P55211 |
Molecular Weight | Predicted: 46kD Observed: 46 kD |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "CASP9 Antibody (OAPB00050)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "CASP9 Antibody (OAPB00050)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "CASP9 Antibody (OAPB00050)" provided in?
This item is provided in "Liquid. Caspase-9 Antibody is supplied in PBS containing 0.02% sodium azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "CASP9 Antibody (OAPB00050)"?
This target may also be called "APAF3;APAF-3;apoptotic protease activating factor 3;apoptotic protease MCH-6;Apoptotic protease-activating factor 3;caspase 9, apoptosis-related cysteine peptidase;caspase-9;ICE-LAP6;ICE-like apoptotic protease 6;MCH6;PPP1R56;protein phosphatase 1, regulatory subunit 56." in publications.
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What is the shipping cost for "CASP9 Antibody (OAPB00050)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "CASP9 Antibody (OAPB00050)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "CASP9 Antibody (OAPB00050)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "Predicted: 46kD Observed: 46 kD".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "CASP9 Antibody (OAPB00050)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "CASP9"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "CASP9"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "CASP9"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "CASP9"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "CASP9"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "CASP9"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.