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Datasheets/Manuals | Printable datasheet for CASP9 Antibody (OAPB00049) (OAPB00049) |
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Predicted Species Reactivity | Human|Mouse|Rat |
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Product Format | Liquid. Caspase-9 Antibody is supplied in PBS containing 0.02% sodium azide. |
Clonality | Polyclonal |
Host | Rabbit |
Conjugation | Unconjugated |
Application | Enzyme-linked immunosorbent assay|Western blot |
Additional Information | HeLa Cell Lysate (OCPB00181) |
:: | Buffer: Antibody is supplied in PBS containing 0.02% sodium azide. |
Reconstitution and Storage | Caspase-9 antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Immunogen | Anti- Caspase 9 antibody (2071) was raised against a peptide corresponding to 16 amino acids near the amino terminus of human Caspase 9. The immunogen is located within amino acids 30-80 of Caspase 9. |
Purification | Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Human; Mouse; Rat |
Concentration | 1 mg/ml |
Blocking Peptide | For anti-CASP9 (OAPB00049) antibody is Catalog # OEPB02177 |
Specificity | It has no cross response to other members in caspase family. |
Application Info | Caspase-9 antibody can be used for detection of caspase-9 by Western blot at 1:1000 to 1:2000 dilution. A 46 kDa band should be detected. It has no cross response to other members in caspase family. (Optimal dilution should be determined by user) |
Reference | Duan H, Orth K, Chinnaiyan AM, Poirier GG, Froelich CJ, He WW, Dixit VM. ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B. J Biol Chem 1996;271:16720-4 ;Srinivasula SM, Fernandes-Alnemri T, Zangrilli J, Robertson N, Armstrong RC, Wang L, Trapani JA, Tomaselli KJ, Litwack G, Alnemri ES. The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32. J Biol Chem 1996;271:27099-106;Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES, Wang X. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997;91:479-89 ;Pan G, O'Rourke K, Dixit VM. Caspase-9, Bcl-XL, and Apaf-1 form a ternary complex. J Biol Chem 1998;273:5841-5 (RD1299) |
Gene Symbol | CASP9 |
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Gene Full Name | caspase 9 |
Alias Symbols | APAF3;APAF-3;apoptotic protease activating factor 3;apoptotic protease MCH-6;Apoptotic protease-activating factor 3;caspase 9, apoptosis-related cysteine peptidase;caspase-9;ICE-LAP6;ICE-like apoptotic protease 6;MCH6;PPP1R56;protein phosphatase 1, regulatory subunit 56. |
NCBI Gene Id | 842 |
Protein Name | Caspase-9 |
Description of Target | Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). |
Uniprot ID | P55211 |
Molecular Weight | Predicted: 46kD Observed: 46kD |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "CASP9 Antibody (OAPB00049)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "CASP9 Antibody (OAPB00049)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "CASP9 Antibody (OAPB00049)" provided in?
This item is provided in "Liquid. Caspase-9 Antibody is supplied in PBS containing 0.02% sodium azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "CASP9 Antibody (OAPB00049)"?
This target may also be called "APAF3;APAF-3;apoptotic protease activating factor 3;apoptotic protease MCH-6;Apoptotic protease-activating factor 3;caspase 9, apoptosis-related cysteine peptidase;caspase-9;ICE-LAP6;ICE-like apoptotic protease 6;MCH6;PPP1R56;protein phosphatase 1, regulatory subunit 56." in publications.
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What is the shipping cost for "CASP9 Antibody (OAPB00049)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "CASP9 Antibody (OAPB00049)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "CASP9 Antibody (OAPB00049)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "Predicted: 46kD Observed: 46kD".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "CASP9 Antibody (OAPB00049)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "CASP9"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "CASP9"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "CASP9"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "CASP9"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "CASP9"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "CASP9"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.