CASP7 Antibody - N-terminal region (ARP58995_P050)

Catalog No: ARP58995_P050

WB Suggested Anti-CASP7 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: OVCAR-3 cell lysateCASP7 is supported by BioGPS gene expression data to be expressed in OVCAR3

Sample: SH-SY5Y cells,  2.0x10⁵ cells 
Primary dilution: 1:2500
Secondary Antibody: HRP-conjugated goat anti-rabbit IgG
Secondary dilution: 1:5000
Image Submitted by: Atsuhiro Tanabe
Pharma Sci Kitasato University 

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Pig, Rabbit, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CASP7
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Goat: 93%; Guinea Pig: 86%; Horse: 100%; Human: 100%; Mouse: 86%; Pig: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%
• Peptide Sequence: Synthetic peptide located within the following region: NNKNFDKVTGMGVRNGTDKDAEALFKCFRSLGFDVIVYNDCSCAKMQDLL
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-CASP7 (ARP58995_P050) antibody is Catalog # AAP58995 (Previous Catalog # AAPP45742)
• Sample Type Confirmation:

  CASP7 is supported by BioGPS gene expression data to be expressed in OVCAR3

Target Info

• Gene Symbol: CASP7
• Gene Full Name: Caspase 7, apoptosis-related cysteine peptidase
• Alias Symbols: MCH3, CMH-1, LICE2, CASP-7, ICE-LAP3
• NCBI Gene Id: 840
• Protein Name: Caspase-7
• Description of Target: This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of this caspase is cleaved by caspase 3 and 10. It is activated upon cell death stimuli and induces apoptosis. Alternative splicing results in four transcript variants, encoding three distinct isoforms.
• Uniprot ID: P55210
• Protein Accession #: NP_203124
• Nucleotide Accession #: NM_033338
• Protein Size (# AA): 336
• Molecular Weight: 37kDa
• Protein Interactions: PTGES3; BAX; PARP1; SAT1; CRYAB; XIAP; RPS6KA1; CTSA; PEPD; MVD; GNS; GBP2; EEF2; CAPN1; CNDP2; GINS2; EIF4H; GZMB; ROCK1; DFFA; CASP9; CASP7; CASP3; ARHGDIA; VIM; STAT1; SPTAN1; CLSPN; PLA2G4B; RB1; UBC; BIRC2; CCT2; VCP; SP1; BIRC3; BIRC6; COPS6; TERF1;