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Catalog No: OKCD00325 (Formerly GWB-KBBTA3)
Size:96WELLS
Price: $675.00
SKU
OKCD00325
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Datasheets/ManualsPrintable datasheet for CAPS ELISA Kit (Human) (OKCD00325)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcyphosine (CAPS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calcyphosine (CAPS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcyphosine (CAPS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Calcyphosine (CAPS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calcyphosine (CAPS) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calcyphosine (CAPS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CAPS Microplate96 Wells (12 x 8 Well Strips)
CAPS Lyophilized Standard2 x 10 ng
100X Biotinylated CAPS Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.052 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Calcyphosine (CAPS). No significant cross-reactivity or interference between Calcyphosine (CAPS) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCAPS
Gene Full Namecalcyphosine
Alias Symbolscalcyphosin;Calcyphosine;calcyphosine 1;CAPS1;epididymis secretory sperm binding protein;thyroid protein p24.
NCBI Gene Id828
Protein NameCalcyphosin
Description of TargetCalcium-binding protein. May play a role in cellular signaling events (Potential).
Uniprot IDQ13938
Protein Accession #NP_004049
Nucleotide Accession #NM_004058.4
Protein Size (# AA)189
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