CAPN3 ELISA Kit (Human) (OKCD02417)

Catalog No: OKCD02417

Size:96WELLS

Human CAPN3 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calpain 3 (CAPN3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calpain 3 (CAPN3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calpain 3 (CAPN3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Calpain 3 (CAPN3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calpain 3 (CAPN3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calpain 3 (CAPN3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CAPN3 Microplate: 96 Wells (12 x 8 Well strips)
  • CAPN3 Lyophilized Standard: 2
  • 100X Biotinylated CAPN3 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids
• Sensitivity: 0.125 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Calpain 3 (CAPN3). No significant cross-reactivity or interference between Calpain 3 (CAPN3) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CAPN3
• Gene Full Name: calpain 3
• Alias Symbols: Calcium-activated neutral proteinase 3;Calpain L3;Calpain p94;calpain p94, large [catalytic] subunit;calpain, large polypeptide L3;calpain-3;CANP3;CANPL3;LGMD2;LGMD2A;LGMDD4;LGMDR1;Muscle-specific calcium-activated neutral protease 3;muscle-specific calcium-activated neutral protease 3 large subunit;nCL-1;new calpain 1;p94.
• NCBI Gene Id: 825
• Protein Name: Calpain-3
• Description of Target: Calcium-regulated non-lysosomal thiol-protease.
• Uniprot ID: P20807
• Protein Accession #: NP_000061.1
• Nucleotide Accession #: NM_000070.2
• Protein Size (# AA): 821