Catalog No: OKCD02416
Size:96WELLS
Price: $650.00
SKU
OKCD02416
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
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- $55: Antibody & Protein in US
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Datasheets/Manuals | Printable datasheet for CAPN1 ELISA Kit (Mouse) (OKCD02416) |
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Predicted Species Reactivity | Mouse|Mus musculus | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calpain 1 (CAPN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calpain 1 (CAPN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calpain 1 (CAPN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Calpain 1 (CAPN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 1.56-100ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calpain 1 (CAPN1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calpain 1 (CAPN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates, cell lysates, cell culture supernatant and other biological fluids | ||||||||||||||||||||||
Sensitivity | 0.61 ng/mL | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of Calpain 1, Large Subunit (CAPN1). No significant cross-reactivity or interference between Calpain 1, Large Subunit (CAPN1) and analogues was observed. | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA | ||||||||||||||||||||||
Protocol Information |
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Gene Symbol | Capn1 |
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Gene Full Name | calpain 1 |
Alias Symbols | Ca;calcium-activated neutral proteinase 1;calpain mu-type;calpain-1 catalytic subunit;calpain-1 large subunit;CANP 1;Cap;Capa1;Capa-1;micromolar-calpain;mu-c;mu-calpain;mu-calpin;muCANP. |
NCBI Gene Id | 12333 |
Protein Name | Calpain-1 catalytic subunit |
Description of Target | Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. |
Uniprot ID | O35350 |
Protein Accession # | NP_001103974.1 |
Nucleotide Accession # | NM_001110504.1 |
Protein Size (# AA) | 713 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
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