Catalog No: OKCD02416
Size:96WELLS
Price: $650.00
SKU
OKCD02416
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Datasheets/ManualsPrintable datasheet for CAPN1 ELISA Kit (Mouse) (OKCD02416)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calpain 1 (CAPN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calpain 1 (CAPN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calpain 1 (CAPN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Calpain 1 (CAPN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range1.56-100ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calpain 1 (CAPN1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calpain 1 (CAPN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CAPN1 Microplate96 Wells (12 x 8 Well strips)
CAPN1 Lyophilized Standard2
100X Biotinylated CAPN1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates, cell culture supernatant and other biological fluids
Sensitivity0.61 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Calpain 1, Large Subunit (CAPN1). No significant cross-reactivity or interference between Calpain 1, Large Subunit (CAPN1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolCapn1
Gene Full Namecalpain 1
Alias SymbolsCa;calcium-activated neutral proteinase 1;calpain mu-type;calpain-1 catalytic subunit;calpain-1 large subunit;CANP 1;Cap;Capa1;Capa-1;micromolar-calpain;mu-c;mu-calpain;mu-calpin;muCANP.
NCBI Gene Id12333
Protein NameCalpain-1 catalytic subunit
Description of TargetCalcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
Uniprot IDO35350
Protein Accession #NP_001103974.1
Nucleotide Accession #NM_001110504.1
Protein Size (# AA)713
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