CANX ELISA Kit (Human) (OKCD00229)

Catalog No: OKCD00229 (Formerly GWB-KBBJN2)

Size:96WELLS

Human CANX ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calnexin (CNX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calnexin (CNX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calnexin (CNX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Calnexin (CNX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calnexin (CNX) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calnexin (CNX) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CANX Microplate: 96 Wells (12 x 8 Well Strips)
  • CANX Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated CANX Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.054 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Calnexin (CNX). No significant cross-reactivity or interference between Calnexin (CNX) and analogues was observed.
• Publications:

  Zhenhua Du, et al. Quantitative proteomics identifies a plasma multi-protein model for detection of hepatocellular carcinoma. 15552 (2020). 32968147

• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Description:
  View product citations for CNX ELISA kit (Human) on CiteAb

Target Info

• Gene Symbol: CANX
• Gene Full Name: calnexin
• Alias Symbols: calnexin;CNX;epididymis secretory sperm binding protein;IP90;major histocompatibility complex class I antigen-binding protein p88;P90.
• NCBI Gene Id: 821
• Protein Name: Calnexin
• Description of Target: Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.
• Uniprot ID: P27824
• Protein Accession #: NP_001019820
• Nucleotide Accession #: NM_001024649.1
• Protein Size (# AA): 592