CAMP ELISA Kit (Mouse) (OKEH03274)

Catalog No: OKEH03274

Size:96T

Mouse CAMP ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology CAMP ELISA Kit (Mouse) (OKEH03274) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Mouse Camp antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Camp and incubated. The Camp found in the sample or standards competes with the biotinylated Camp for limited binding sites on the immobilized anti-Mouse Camp antibody. Excess unbound biotinylated Camp and sample or standard Camp is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Camp captured in the well and inversely proportional to the amount of Camp which was contained in the sample or standard.
• ELISA Kit Range: 0.156-10ng/ml
• ELISA Kit Recovery: Spike Average Recovery: 80%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 5.1% (n = 20)
  Mean Inter-assay CV%: <= 7.2% (n = 20)
• ELISA Kit Component:
  • Anti-Camp Microplate: 96 Wells (12 x 8 Well strips)
  • Camp Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Camp-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.091 ng/mL
• Specificity: Natural and recombinant Mouse Cathelin-related antimicrobial peptide
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: Camp
• Gene Full Name: cathelicidin antimicrobial peptide
• Alias Symbols: C;CAP;CAP18;cathelicidin antimicrobial peptide;cathelin-like protein;cathelin-related antimicrobial peptide;CLP;Cnlp;Cr;Cramp;FAL;FALL39;MC;MCLP.
• NCBI Gene Id: 12796
• Protein Name: P51437
• Description of Target: Cathelicidin antimicrobial peptide
• Uniprot ID: https://www.uniprot.org/uniprot/P51437
• Protein Accession #: NP_034051.2
• Nucleotide Accession #: NM_009921.2
• Protein Size (# AA): 172
• Molecular Weight: 19 kDa