Catalog No: OKCD00809 (Formerly GWB-KBBEC7)
Size:96WELLS
Price: $650.00
SKU
OKCD00809
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Datasheets/ManualsPrintable datasheet for CAMK2G ELISA Kit (Rat) (OKCD00809)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Camk2g Microplate96 Wells (12 x 8 Well Strips)
Camk2g Lyophilized Standard2 x 20 ng
100X Biotinylated Camk2g Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids.
Sensitivity< 0.118 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g). No significant cross-reactivity or interference between Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCamk2g
Gene Full Namecalcium/calmodulin-dependent protein kinase II gamma
Alias SymbolsCa+2/calmodulin-dependent protein kinase II gamma;calcium/calmodulin-dependent protein kinase (CaM kinase) II gamma;calcium/calmodulin-dependent protein kinase type II gamma chain;calcium/calmodulin-dependent protein kinase type II subunit gamma;calcium/calmodulin-dependent protein kinase type II subunit gamma-like;caM kinase II subunit gamma;caMK-II subunit gamma;caM-kinase II gamma chain.
NCBI Gene Id171140
Protein NameCalcium/calmodulin-dependent protein kinase type II subunit gamma
Description of TargetCalcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in sarcoplasmic reticulum Ca(2+) transport in skeletal muscle and may function in dendritic spine and synapse formation and neuronal plasticity. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR through phosphorylation of the ryanodine receptor-coupling factor triadin. In neurons, may participate in the promotion of dendritic spine and synapse formation and maintenance of synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning (By similarity).
Uniprot IDP11730
Protein Accession #NP_598289
Nucleotide Accession #NM_133605.1
Protein Size (# AA)527
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