CAMK2A ELISA Kit (Mouse) (OKCD02414)

Catalog No: OKCD02414

Size:96WELLS

Mouse CAMK2A ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  	95-103%	87-103%	98-105%	79-89%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  	82-92	88

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CAMK2A Microplate: 96 Wells (12 x 8 Well strips)
  • CAMK2A Lyophilized Standard: 2
  • 100X Biotinylated CAMK2A Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: 0.112 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a). No significant cross-reactivity or interference between Calcium/Calmodulin Dependent Protein Kinase II Alpha (CAMK2a) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Camk2a
• Gene Full Name: calcium/calmodulin-dependent protein kinase II alpha
• Alias Symbols: alpha-CaMKII;calcium/calmodulin-dependent protein kinase type II subunit alpha;caM kinase II subunit alpha;CaMK II;CaMKII;caMK-II subunit alpha;mKIAA0968;R74975.
• NCBI Gene Id: 12322
• Protein Name: Calcium/calmodulin-dependent protein kinase type II subunit alpha
• Description of Target: CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. Phosphorylates transcription factor FOXO3 on 'Ser-298' (By similarity). Activates FOXO3 transcriptional activity (PubMed:23805378).
• Uniprot ID: P11798
• Protein Accession #: NP_001273738.1
• Nucleotide Accession #: NM_001286809.1
• Protein Size (# AA): 478