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Catalog No: OKEH02667
Size:96T
Price: $525.00
SKU
OKEH02667
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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CALM1 ELISA Kit (Human) : 96 Wells (OKEH02667)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CALM1 ELISA Kit (Human) (OKEH02667) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CALM1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for CALM1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CALM1 captured in well.
ELISA Kit Range1.56-100ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.1% (n = 20)
Mean Inter-assay CV%: < 7.9% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-CALM1 Microplate96 Wells (12 x 8 Well strips)
CALM1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated CALM1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.99 ng/mL
SpecificityNatural and recombinant Human Calmodulin
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCALM1, CALM2, CALM3
Gene Full Namecalmodulin 1, calmodulin 2, calmodulin 3
Alias SymbolsCALM;CALML2;calmodulin 1 (phosphorylase kinase, delta);calmodulin 2 (phosphorylase kinase, delta);calmodulin-1;Calmodulin-2;Calmodulin-3;caM;CAM1;CAM2;CAM3;CAMB;CAMC;CAMI;CAMII;CAMIII;CPVT4;CPVT6;DD132;epididymis secretory protein Li 72;HEL-S-72;LP7057 protein;LQT14;LQT15;LQT16;PHKD;PHKD2;PHKD3;phosphorylase kinase delta;phosphorylase kinase subunit delta;phosphorylase kinase, delta subunit;prepro-calmodulin 1;prepro-calmodulin 2;prepro-calmodulin 3.
NCBI Gene Id801, 805, 808
Protein NameP0DP23
Description of TargetCalmodulin-1
Uniprot IDhttps://www.uniprot.org/uniprot/P0DP23
Protein Accession #NP_008819.1
Nucleotide Accession #NM_006888.4
Protein Size (# AA)149
Molecular Weight17 kDa
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