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Catalog No: OKEH03974
Size:96T
Price: $675.00
SKU
OKEH03974
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityChicken, Gallus gallus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CALB1 ELISA Kit (Chicken) (OKEH03974) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CALB1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CALB1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CALB1 captured in well.
ELISA Kit Range0.625-40ng/mL
ELISA Kit Component
ComponentAmount
CALB1 Microplate96 Wells (12 x 8 Well strips)
CALB1 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated CALB1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.271 ng/mL
SpecificityNatural and recombinant Chicken Calbindin
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCALB1
Gene Full Namecalbindin 1
Alias Symbols28-kDa vitamin D-dependent calcium-binding protein;CaBP-28;CaBP-D28k;calbindin;calbindin 1, 28kDa;calbindin D28;Calbindin D28k;Calbindin D-28K;Chicken intestinal 28-kilodalton calbindin-D;D-28K;vitamin D-dependent calcium-binding protein, avian-type;vitamin D-induced calbindin-D28K.
NCBI Gene Id396519
Protein NameP04354
Description of TargetCalbindin
Uniprot IDhttps://www.uniprot.org/uniprot/P04354
Protein Accession #NP_990844.1
Nucleotide Accession #NM_205513.1
Protein Size (# AA)262
Molecular Weight262 kDa
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