Catalog No: OKCD00963 (Formerly GWB-KBBDJ3)
Size:96WELLS
Price: $700.00
SKU
OKCD00963
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Datasheets/ManualsPrintable datasheet for CACNA1C ELISA Kit (Mouse) (OKCD00963)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNa1C). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNa1C). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNa1C), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNa1C) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNa1C) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNa1C) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Cacna1c Microplate96 Wells (12 x 8 Well Strips)
Cacna1c Lyophilized Standard2 x 20 ng
100X Biotinylated Cacna1c Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.115 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNa1C). No significant cross-reactivity or interference between Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNa1C) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCacna1c
Gene Full Namecalcium channel, voltage-dependent, L type, alpha 1C subunit
Alias Symbolsbrain class C;calcium channel voltage-dependent alpha1c subunit;Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle;Cav1;Cav1.2;Cchl1;Cchl1a1;D930026N18Rik;MBC;MELC-CC;Mouse brain class C;neuronal voltage-gated calcium channel alpha 1C subunit;skeletal muscle-specific calcium channel;voltage-dependent L-type calcium channel subunit alpha-1C;voltage-gated calcium channel subunit alpha Cav1.2.
NCBI Gene Id12288
Protein NameVoltage-dependent L-type calcium channel subunit alpha-1C
Description of TargetVoltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1C gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing the alpha-1C subunit play an important role in excitation-contraction coupling in the heart. The various isoforms display marked differences in the sensitivity to DHP compounds. Binding of calmodulin or CABP1 at the same regulatory sites results in opposite effects on the channel function.
Uniprot IDQ01815
Protein Accession #NP_001153005
Nucleotide Accession #NM_001159533.2
Protein Size (# AA)2139
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