Catalog No: OKCD00963 (Formerly GWB-KBBDJ3)
Size:96WELLS
Price: $700.00
SKU
OKCD00963
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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Datasheets/Manuals | Printable datasheet for CACNA1C ELISA Kit (Mouse) (OKCD00963) |
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Predicted Species Reactivity | Mouse|Mus musculus | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNa1C). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNa1C). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNa1C), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNa1C) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNa1C) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNa1C) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates and other biological fluids. | ||||||||||||||||||||||
Sensitivity | < 0.115 ng/mL | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNa1C). No significant cross-reactivity or interference between Calcium Channel, Voltage Dependent, L-Type, Alpha 1C Subunit (CACNa1C) and analogues was observed. | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich Immunoassay |
Gene Symbol | Cacna1c |
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Gene Full Name | calcium channel, voltage-dependent, L type, alpha 1C subunit |
Alias Symbols | brain class C;calcium channel voltage-dependent alpha1c subunit;Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle;Cav1;Cav1.2;Cchl1;Cchl1a1;D930026N18Rik;MBC;MELC-CC;Mouse brain class C;neuronal voltage-gated calcium channel alpha 1C subunit;skeletal muscle-specific calcium channel;voltage-dependent L-type calcium channel subunit alpha-1C;voltage-gated calcium channel subunit alpha Cav1.2. |
NCBI Gene Id | 12288 |
Protein Name | Voltage-dependent L-type calcium channel subunit alpha-1C |
Description of Target | Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1C gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing the alpha-1C subunit play an important role in excitation-contraction coupling in the heart. The various isoforms display marked differences in the sensitivity to DHP compounds. Binding of calmodulin or CABP1 at the same regulatory sites results in opposite effects on the channel function. |
Uniprot ID | Q01815 |
Protein Accession # | NP_001153005 |
Nucleotide Accession # | NM_001159533.2 |
Protein Size (# AA) | 2139 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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