CACNA1A ELISA Kit (Human) : 96 Wells (OKEH00960)

Catalog No: OKEH00960

Size:96T

Human CACNA1A ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology CACNA1A ELISA Kit (Human) (OKEH00960) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CACNA1A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CACNA1A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CACNA1A captured in well.
  
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.2% (n = 20)
  Mean Inter-assay CV%: < 9.1% (n = 20)
• ELISA Kit Component:
  • CACNA1A Microplate: 96 Wells (12 x 8 Well strips)
  • CACNA1A Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated CACNA1A Detector Antibody: 1 x 120 ul
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.078 ng/mL
• Specificity: Natural and recombinant Human Voltage-dependent P/Q-type calcium channel subunit alpha-1A
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CACNA1A
• Gene Full Name: calcium voltage-gated channel subunit alpha1 A
• Alias Symbols: APCA;BI;brain calcium channel 1;brain calcium channel I;CACNL1A4;calcium channel, L type, alpha-1 polypeptide;Calcium channel, L type, alpha-1 polypeptide isoform 4;calcium channel, voltage-dependent, P/Q type, alpha 1A subunit;CAV2.1;DEE42;EA2;EIEE42;fetal brain Ca2+ voltage-gated channel alpha1A pore-forming subunit;FHM;HPCA;MHP;MHP1;SCA6;voltage-dependent P/Q-type calcium channel subunit alpha-1A;voltage-gated calcium channel subunit alpha Cav2.1.
• NCBI Gene Id: 773
• Protein Name: O00555
• Description of Target: Voltage-dependent P/Q-type calcium channel subunit alpha-1A
• Uniprot ID: https://www.uniprot.org/uniprot/O00555
• Protein Accession #: NP_000059.3
• Nucleotide Accession #: NM_000068.3
• Protein Size (# AA): 2505
• Molecular Weight: 282 kDa