CA9 ELISA Kit (Human) : 96 Wells (OKEH02665)

Catalog No: OKEH02665

Size:96T

Human CA9 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology CA9 ELISA Kit (Human) (OKEH02665) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CA9 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for CA9 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CA9 captured in well.
• ELISA Kit Range: 15.6-1000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 95%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.4% (n = 20)
  Mean Inter-assay CV%: < 7.2% (n = 20)
• ELISA Kit Component:
  • Anti-CA9 Microplate: 96 Wells (12 x 8 Well strips)
  • CA9 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated CA9 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 7.9 pg/mL
• Specificity: Natural and recombinant Human Carbonic anhydrase 9
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CA9
• Gene Full Name: carbonic anhydrase 9
• Alias Symbols: CAIX;CA-IX;carbonate dehydratase IX;carbonic anhydrase 9;carbonic anhydrase IX;carbonic dehydratase;membrane antigen MN;MN;P54/58N;pMW1;RCC-associated antigen G250;RCC-associated protein G250;renal cell carcinoma-associated antigen G250.
• NCBI Gene Id: 768
• Protein Name: Q16790
• Description of Target: Carbonic anhydrase 9
• Uniprot ID: https://www.uniprot.org/uniprot/Q16790
• Protein Accession #: NP_001207.2
• Nucleotide Accession #: NM_001216.2
• Protein Size (# AA): 459
• Molecular Weight: 50 kDa