Catalog No: OKEH02399
Size:96T
Price: $475.00
SKU
OKEH02399
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.

C9 ELISA Kit (Human) : 96 Wells (OKEH02399)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology C9 ELISA Kit (Human) (OKEH02399) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for C9 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for C9 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample C9 captured in well.
ELISA Kit Range0.312-20ng/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 86%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.8% (n = 20)
Mean Inter-assay CV%: < 7.9% (n = 20)
ELISA Kit Component
ComponentAmount
C9 Microplate96 Wells (12 x 8 Well strips)
C9 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated S100A1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.12 ng/mL
SpecificityNatural and recombinant Human Complement component C9
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolC9
Gene Full Namecomplement C9
Alias SymbolsARMD15;C9D;complement component 9;complement component C9.
NCBI Gene Id735
Protein NameP02748
Description of TargetComplement component C9
Uniprot IDhttps://www.uniprot.org/uniprot/P02748
Protein Accession #NP_001728.1
Nucleotide Accession #NM_001737.3
Protein Size (# AA)559
Molecular Weight63 kDa
Write Your Own Review
You're reviewing:C9 ELISA Kit (Human) : 96 Wells (OKEH02399)
Your Rating
We found other products you might like!