Catalog No: OKEH01921
Size:96T
Price: $600.00
SKU
OKEH01921
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology C8B ELISA Kit (Human) (OKEH01921) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for C8B has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for C8B is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample C8B captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.9% (n = 20)
Mean Inter-assay CV%: < 8.5% (n = 20)
ELISA Kit Component
ComponentAmount
C8B Microplate96 Wells (12 x 8 Well strips)
C8B Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated C8B Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.056 ng/mL
SpecificityNatural and recombinant Human Complement component C8 beta chain
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolC8B
Gene Full Namecomplement C8 beta chain
Alias SymbolsC82;complement component 8 subunit beta;complement component 8, beta polypeptide;complement component C8 beta chain.
NCBI Gene Id732
Protein NameP07358
Description of TargetComplement component C8 beta chain
Uniprot IDhttps://www.uniprot.org/uniprot/P07358
Protein Accession #NP_000057.2
Nucleotide Accession #NM_000066.3
Protein Size (# AA)591
Molecular Weight67 kDa
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