Catalog No: OKCD01374
Size:96 Wells
Price: $625.00
SKU
OKCD01374
Availability: Discontinued
Please contact customer service when ordering 10+ kits.
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C5b-9 High Sensitivity ELISA Kit (Mouse) (OKCD01374)
Predicted Species Reactivity | Mouse | ||||||||||||||||||||||
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ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | 2.5 Hours | ||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | Aviva Systems Biology C5b-9 High Sensitivity ELISA Kit (Mouse) (OKCD01374) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for C5b-9 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for C5b-9 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample C5b-9 captured in well. | ||||||||||||||||||||||
ELISA Kit Range | 1.56 - 100 ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Intra-assay Precision: 3 samples with known low, middle and high level C5b-9 were tested with 20 replicates across one plate. Inter-assay Precision: 3 samples with known low, middle and high level C5b-9 were tested on three different plates with 8 replicates, respectively. Intra-Assay CV: <10% Inter-Assay CV: <12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Plasma, tissue homogenates and other biological fluids | ||||||||||||||||||||||
Sensitivity | < 0.63 ng/mL | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of Terminal Complement Complex C5b-9 (C5b-9). No significant cross-reactivity or interference between Terminal Complement Complex C5b-9 (C5b-9) and analogues was observed. | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | C5B-9 |
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Alias Symbols | Terminal Complement Complex C5b-9 |
Protein Name | Terminal Complement Complex C5b-9 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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