Catalog No: OKCD01374
Size:96 Wells
Price: $625.00
SKU
OKCD01374
Availability: Discontinued
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit Linearity
Sample1:21:41:81:16
EDTA Plasma (n=5)80-101%82-98%88-96%87-101%
ELISA Kit PrincipleAviva Systems Biology C5b-9 High Sensitivity ELISA Kit (Mouse) (OKCD01374) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for C5b-9 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for C5b-9 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample C5b-9 captured in well.
ELISA Kit Range1.56 - 100 ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
EDTA Plasma (n=5)84-9692
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level C5b-9 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level C5b-9 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
ELISA Kit Component
ComponentAmount
Anti-C5b-9 Microplate96 Wells (12 x 8 Well Strips)
C5b-9 Lyophilized Standard2
100X Biotinylated C5b-9 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypePlasma, tissue homogenates and other biological fluids
Sensitivity< 0.63 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Terminal Complement Complex C5b-9 (C5b-9). No significant cross-reactivity or interference between Terminal Complement Complex C5b-9 (C5b-9) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolC5B-9
Alias SymbolsTerminal Complement Complex C5b-9
Protein NameTerminal Complement Complex C5b-9
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