C5 Antibody (OAGA00917)

Catalog No: OAGA00917

Size:100 ul

Sample (30 ug of whole cell lysate)
A: H1299
5% SDS PAGE
OAGA00917 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded hepatoma, using C5 antibody(OAGA00917) antibody at 1:500 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IHC-FFPE, WB
• Additional Information: Background: The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to a susceptibility to liver fibrosis and to rheumatoid arthritis. [provided by RefSeq]
  Positive Controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji
  Cellular Localization: Secreted
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1223 and 1451 of C5 (Uniprot ID#P01031)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: CPAMD4, FLJ17816, FLJ17822, MGC142298, C5
• Gene Full Name: Complement C5
• Alias Symbols: C5D, C5a, C5b, ECLZB, CPAMD4
• NCBI Gene Id: 727
• Protein Name: Complement C5
• Uniprot ID: P01031
• Protein Accession #: NP_001726.2
• Molecular Weight: 188 kDa