C1QTNF4 ELISA Kit (Human) (OKCA01170)

Catalog No: OKCA01170

Size:96 Wells

Human C1QTNF4 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  Neat	98	94-103
  1:2	85	82-91
  1:4	103	100-105
  1:8	88	84-95

• ELISA Kit Principle: Aviva Systems Biology C1QTNF4 ELISA Kit (Human) (OKCA01170) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for C1QTNF4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for C1QTNF4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample C1QTNF4 captured in the well.
• ELISA Kit Range: 62.5 - 4,000 pg/mL
• ELISA Kit Recovery:

  Sample	Mean Recovery (%)	Range (%)
  Serum (n=5)	100	95-105
  EDTA Plasma (n=4)	86	83-89

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level OKCA01170 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level OKCA01170 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • C1QTNF4 Microplate: 96 Wells (12 x 8 Well strips)
  • C1QTNF4 Lyophilized Standard: 2 x 4 ng
  • 100X Biotinylated C1QTNF4 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Sensitivity: 15.6 pg/mL
• Assay Info: Assay Methodology: Quantitative Sanadwich ELISA

Target Info

• Gene Symbol: C1QTNF4
• Gene Full Name: C1q and tumor necrosis factor related protein 4
• Alias Symbols: CTRP4, ZACRP4
• NCBI Gene Id: 114900
• Protein Name: Complement C1q tumor necrosis factor-related protein 4
• Description of Target: May act as a pro-inflammatory cytokine, that induces the activation of NF-kappa-B signaling pathway and up-regulates IL6 production.
• Uniprot ID: Q9BXJ3
• Protein Accession #: NP_114115.2
• Nucleotide Accession #: NM_031909.2