C1QA ELISA Kit (Rat) (OKCD02316)

Catalog No: OKCD02316

Size:96WELLS

Rat C1QA ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	95-102%	78-94%	92-102%	95-102%
  EDTA Plasma (n=5)	89-104%	97-105%	88-102%	88-102%
  Heparin Plasma (n=5)	78-97%	79-93%	78-98%	80-103%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 1, Q Subcomponent A (C1qA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Component 1, Q Subcomponent A (C1qA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Component 1, Q Subcomponent A (C1qA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Complement Component 1, Q Subcomponent A (C1qA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	79-88	80
  EDTA Plasma (n=5)	78-104	93
  Heparin Plasma(n=5)	86-104	92

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Component 1, Q Subcomponent A (C1qA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Component 1, Q Subcomponent A (C1qA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-C1QA Microplate: 96 Wells (12 x 8 Well strips)
  • C1QA Lyophilized Standard: 2
  • 100X Biotinylated C1QA Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.67 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Complement Component 1, Q Subcomponent A (C1qA). No significant cross-reactivity or interference between Complement Component 1, Q Subcomponent A (C1qA) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: C1qa
• Gene Full Name: complement C1q A chain
• Alias Symbols: Adic;adiponectin c;complement C1q subcomponent subunit A;complement component 1, q subcomponent, A chain;complement component 1, q subcomponent, alpha polypeptide.
• NCBI Gene Id: 298566
• Protein Name: Complement C1q subcomponent subunit A
• Description of Target: C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
• Uniprot ID: P31720
• Protein Accession #: NP_001008515.1
• Nucleotide Accession #: NM_001008515.1
• Protein Size (# AA): 245