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Catalog No: OKEH03210
Size:96T
Price: $500.00
SKU
OKEH03210
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology C1QA ELISA Kit (Mouse) (OKEH03210) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for C1qa has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for C1qa is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample C1qa captured in well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 86%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 4.8% (n = 20)
Mean Inter-assay CV%: <= 7.1% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-C1qa Microplate96 Wells (12 x 8 Well strips)
C1qa Lyophilized Standard2
100X Biotinylated C1qa Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity7.10 pg/mL
SpecificityNatural and recombinant Mouse Complement C1q subcomponent subunit A
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolC1qa
Gene Full Namecomplement component 1, q subcomponent, alpha polypeptide
Alias SymbolsAdic;adiponectin c;AI255395;C1q;complement C1q subcomponent subunit A.
NCBI Gene Id12259
Protein NameP98086
Description of TargetComplement C1q subcomponent subunit A
Uniprot IDhttps://www.uniprot.org/uniprot/P98086
Protein Accession #NP_031598.2
Nucleotide Accession #NM_007572.2
Protein Size (# AA)245
Molecular Weight26 kDa
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