BUB1B Antibody (OAAF02041)

Catalog No: OAAF02041 (Formerly GWB-ASD314)

Size:100 ug

Western blot analysis of extracts from HeLa/HepG2 cells, treated with H2O2 100uM 30', using BUB1B Antibody. The lane on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human BUB1B.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: BUB1B antibody detects endogenous levels of total BUB1B protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:3000; ELISA: 1:20000
• Reference: Taylor S.S., J. Cell Biol. 142:1-11(1998).
  Chan G.K.T., J. Cell Biol. 143:49-63(1998).
  Cahill D.P., Nature 392:300-303(1998).

Target Info

• Gene Symbol: BUB1B
• Gene Full Name: BUB1 mitotic checkpoint serine/threonine kinase B
• Alias Symbols: Bub1A;BUB1B, mitotic checkpoint serine/threonine kinase;BUB1beta;BUBR1;budding uninhibited by benzimidazoles 1 homolog beta;hBUBR1;MAD3/BUB1-related protein kinase;MAD3L;mitotic checkpoint kinase MAD3L;mitotic checkpoint serine/threonine-protein kinase BUB1 beta;MVA1;Protein SSK1;SSK1.
• NCBI Gene Id: 701
• Protein Name: Mitotic checkpoint serine/threonine-protein kinase BUB1 beta
• Description of Target: Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.
• Uniprot ID: O60566
• Protein Accession #: NP_001202.4
• Molecular Weight: 119 kDa