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Catalog No: OKEH03136
Size:96T
Price: $525.00
SKU
OKEH03136
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology BTNL2 ELISA Kit (Human) (OKEH03136) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human BTNL2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated BTNL2 and incubated. The BTNL2 found in the sample or standards competes with the biotinylated BTNL2 for limited binding sites on the immobilized anti-human BTNL2 antibody. Excess unbound biotinylated BTNL2 and sample or standard BTNL2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated BTNL2 captured in the well and inversely proportional to the amount of BTNL2 which was contained in the sample or standard.
ELISA Kit Range0.312-20ng/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 7.9% (n = 20)
Mean Inter-assay CV%: <= 9.2% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-BTNL2 Microplate96 Wells (12 x 8 Well strips)
BTNL2 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X BTNL2-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.164 ng/mL
SpecificityNatural and recombinant Human Butyrophilin-like protein 2
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolBTNL2
Gene Full Namebutyrophilin like 2
Alias SymbolsBTL-II;BTN7;butyrophilin-like 2 (MHC class II associated);butyrophilin-like protein 2;HSBLMHC1;SS2;truncated BTNL2.
NCBI Gene Id56244
Protein NameQ9UIR0
Description of TargetButyrophilin-like protein 2
Uniprot IDhttps://www.uniprot.org/uniprot/Q9UIR0
Protein Accession #NP_001291490.1
Nucleotide Accession #NM_001304561.1
Protein Size (# AA)455
Molecular Weight50 kDa
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