BRSK1 Antibody (OAAF02566)

Catalog No: OAAF02566

Size:100 ug

Western blot analysis of extracts from 293 cells, using BRSK1 Antibody. The lane on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human BRSK1.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: BRSK1 antibody detects endogenous levels of total BRSK1 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:3000; ELISA: 1:1000
• Reference: Lizcano J.M., EMBO J. 23:833-843(2004).
  Lu R., J. Biol. Chem. 279:31164-31170(2004).
  Nagase T., DNA Res. 8:85-95(2001).

Target Info

• Gene Symbol: BRSK1
• Gene Full Name: BR serine/threonine kinase 1
• Alias Symbols: BR serine/threonine-protein kinase 1;brain-selective kinase 1;brain-specific serine/threonine-protein kinase 1;hSAD1;protein kinase SAD1A;SAD1 homolog;SAD1 kinase;serine/threonine-protein kinase BRSK1;serine/threonine-protein kinase SAD-B;synapses of Amphids Defective homolog 1.
• NCBI Gene Id: 84446
• Protein Name: Serine/threonine-protein kinase BRSK1
• Description of Target: Serine/threonine-protein kinase that plays a key role in polarization of neurons and centrosome duplication. Phosphorylates CDC25B, CDC25C, MAPT/TAU, RIMS1, TUBG1, TUBG2 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-529' and 'Ser-579'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in post-mitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. In neurons, localizes to synaptic vesicles and plays a role in neurotransmitter release, possibly by phosphorylating RIMS1. Also acts as a positive regulator of centrosome duplication by mediating phosphorylation of gamma-tubulin (TUBG1 and TUBG2) at 'Ser-131', leading to translocation of gamma-tubulin and its associated proteins to the centrosome. Involved in the UV-induced DNA damage checkpoint response, probably by inhibiting CDK1 activity through phosphorylation and activation of WEE1, and inhibition of CDC25B and CDC25C.
• Uniprot ID: Q8TDC3
• Protein Accession #: NP_115806.1
• Molecular Weight: 86 kDa