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Catalog No: OKEH04838
Size:96T
Price: $525.00
SKU
OKEH04838
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology BRCA2 ELISA Kit (Human) (OKEH04838) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BRCA2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BRCA2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BRCA2 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
BRCA2 Microplate96 Wells (12 x 8 Well Strips)
BRCA2 Standard2
Sample Diluent1 x 20 mL
BRCA2 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity0.078 ng/mL
SpecificityNatural and recombinant Human Breast cancer type 2 susceptibility protein
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolBRCA2
Gene Full NameBRCA2 DNA repair associated
Alias SymbolsBRCA1/BRCA2-containing complex, subunit 2;BRCC2;breast and ovarian cancer susceptibility gene, early onset;breast and ovarian cancer susceptibility protein 2;breast cancer 2 tumor suppressor;breast cancer 2, early onset;breast cancer type 2 susceptibility protein;BROVCA2;FACD;FAD;FAD1;FANCD;FANCD1;Fanconi anemia group D1 protein;GLM3;mutant BRCA2;PNCA2;truncated BRCA2 DNA repair associated;truncated BRCA2 DNA repair-associated protein;truncated breast cancer 2;truncated DNA repair-associated BRCA2;truncated DNA repair-associated protein;XRCC11.
NCBI Gene Id675
Protein NameP51587
Description of TargetBreast cancer type 2 susceptibility protein
Uniprot IDhttps://www.uniprot.org/uniprot/P51587
Protein Accession #NP_000050.2
Nucleotide Accession #NM_000059.3
Protein Size (# AA)3418
Molecular Weight384 kDa
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