Bradykinin ELISA Kit (Mouse) (OKCD02257)

Catalog No: OKCD02257

Size:96WELLS

Mouse BK ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 2h
• ELISA Kit Linearity:

  Matrix	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	93-105%	84-99%	81-104%	85-105%
  EDTA plasma(n=5)	99-105%	96-103%	93-101%	96-104%
  heparin plasma(n=5)	92-101%	80-98%	97-105%	94-105%

• ELISA Kit Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Bradykinin (BK) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Bradykinin (BK) and unlabeled Bradykinin (BK) (Standards or samples) with the pre-coated antibody specific to Bradykinin (BK). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Bradykinin (BK) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Bradykinin (BK) in the sample.
• ELISA Kit Range: 1.23-100pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	84-98	88
  EDTA plasma(n=5)	88-95	91
  heparin plasma(n=5)	91-105	96

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bradykinin (BK) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bradykinin (BK) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Bradykinin Microplate: 96 Wells (12 x 8 Well Strips)
  • Bradykinin Lyophilized Standard: 2
  • Bradykinin Biotin Complex: 1
  • 100X Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Biotin Complex Diluent: 1 x 12 mL
  • Avidin/HRP Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
  • Biotin Complex Reconstitution Buffer: 1 x 300 uL
• Additional Information: CAS: 58-82-2
  PubChem: 439201
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 0.48 pg/mL
• Assay Info: Assay Methodology: Competitive Inhibition Immunoassay

Target Info

• Gene Symbol: Kng1
• Gene Full Name: kininogen 1
• Alias Symbols: H-kin;H-kininigen;kininogen-1;Kng;L-ki;L-kininogen.
• NCBI Gene Id: 16644
• Protein Name: Kininogen-1
• Description of Target: (1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting (By similarity).
• Uniprot ID: O08677