BPIFA1 ELISA Kit (Human) (OKCD00535)

Catalog No: OKCD00535 (Formerly GWB-KBBIE3)

Size:96WELLS

Human BPIFA1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	90-102%	79-101%	88-99%	78-99%
  EDTA Plasma (n=5)	80-95%	89-102%	91-98%	91-98%
  Heparin Plasma (n=5)	91-99%	94-103%	81-90%	89-103%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Palate/Lung And Nasal Epithelium Associated Protein (PLUNC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Palate/Lung And Nasal Epithelium Associated Protein (PLUNC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Palate/Lung And Nasal Epithelium Associated Protein (PLUNC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Palate/Lung And Nasal Epithelium Associated Protein (PLUNC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	98-105	101
  EDTA plasma(n=5)	94-101	98
  heparin plasma(n=5)	94-101	97

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Palate/Lung And Nasal Epithelium Associated Protein (PLUNC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Palate/Lung And Nasal Epithelium Associated Protein (PLUNC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-BPIFA1 Microplate: 96 Wells (12 x 8 Well Strips)
  • BPIFA1 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated BPIFA1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.057 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Palate/Lung And Nasal Epithelium Associated Protein (PLUNC). No significant cross-reactivity or interference between Palate/Lung And Nasal Epithelium Associated Protein (PLUNC) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: BPIFA1
• Gene Full Name: BPI fold containing family A member 1
• Alias Symbols: bA49G10.5;BPI fold-containing family A member 1;ligand-binding protein RYA3;lung-specific protein X;LUNX;NASG;nasopharyngeal carcinoma-related protein;palate lung and nasal epithelium clone protein;palate, lung and nasal epithelium associated;PLUNC;protein Plunc;secretory protein in upper respiratory tracts;short PLUNC1;SPLUNC1;SPURT;tracheal epithelium enriched protein;Tracheal epithelium-enriched protein;von Ebner protein Hl.
• NCBI Gene Id: 51297
• Protein Name: BPI fold-containing family A member 1
• Description of Target: Plays a role in the innate immune responses of the upper airways. Reduces the surface tension in secretions from airway epithelia and inhibits the formation of biofilm by pathogenic Gram-negative bacteria, such as P.aeruginosa and K.pneumoniae. Binds bacterial lipopolysaccharide (LPS). Negatively regulates proteolytic cleavage of SCNN1G, an event that is required for activation of the epithelial sodium channel (ENaC), and thereby contributes to airway surface liquid homeostasis and proper clearance of mucus. Plays a role in the airway inflammatory response after exposure to irritants. May attract macrophages and neutrophils. May be associated with tumor progression.
• Uniprot ID: Q9NP55
• Protein Accession #: NP_001230122
• Nucleotide Accession #: NM_001243193.1
• Protein Size (# AA): 256