BNIP3 ELISA Kit (Rat) (OKCD01168)

Catalog No: OKCD01168 (Formerly GWB-KBBEE9)

Size:96WELLS

Rat Bnip3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.625-40ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Bnip3 Microplate: 96 Wells (12 x 8 Well Strips)
  • Bnip3 Lyophilized Standard: 2 x 40 ng
  • 100X Biotinylated Bnip3 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.054ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3). No significant cross-reactivity or interference between Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Bnip3
• Gene Full Name: BCL2 interacting protein 3
• Alias Symbols: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3;BCL2/adenovirus E1B 19 kDa-interacting protein 3;BCL2/adenovirus E1B interacting protein 3.
• NCBI Gene Id: 84480
• Protein Name: BCL2-interacting protein 3
• Description of Target: BCL2-interacting protein 3
• Uniprot ID: Q9ET45
• Protein Accession #: NP_445872
• Nucleotide Accession #: NM_053420.3
• Protein Size (# AA): 187