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Catalog No: OKEH01576
Size:96T
Price: $525.00
SKU
OKEH01576
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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BMX ELISA Kit (Human) : 96 Wells (OKEH01576)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology BMX ELISA Kit (Human) (OKEH01576) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BMX has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BMX is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BMX captured in well.
ELISA Kit Range1.56-100ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 3.4% (n = 20)
Mean Inter-assay CV%: < 6.9% (n = 20)
ELISA Kit Component
ComponentAmount
BMX Microplate96 Wells (12 x 8 Well strips)
BMX Lyophilized Standard2
100X Biotinylated BMX Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.39 ng/mL
SpecificityNatural and recombinant Human Cytoplasmic tyrosine-protein kinase BMX
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolBMX
Gene Full NameBMX non-receptor tyrosine kinase
Alias Symbolsbone marrow tyrosine kinase gene in chromosome X protein;BTK-like on X chromosome;cytoplasmic tyrosine-protein kinase BMX;epithelial and endothelial tyrosine kinase;ETK;Etk/Bmx cytosolic tyrosine kinase;NTK38;NTK38 tyrosine kinase;PSCTK2;PSCTK3.
NCBI Gene Id660
Protein NameP51813
Description of TargetCytoplasmic tyrosine-protein kinase BMX
Uniprot IDhttps://www.uniprot.org/uniprot/P51813
Protein Accession #NP_001712.1
Nucleotide Accession #NM_001721.6
Protein Size (# AA)675
Molecular Weight78 kDa
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