BMPR1A ELISA Kit (Mouse) (OKCD02403)

Catalog No: OKCD02403

Size:96WELLS

Mouse BMPR1A ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein Receptor 1A (BMPR1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein Receptor 1A (BMPR1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bone Morphogenetic Protein Receptor 1A (BMPR1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Bone Morphogenetic Protein Receptor 1A (BMPR1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein Receptor 1A (BMPR1A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein Receptor 1A (BMPR1A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-BMPR1A Microplate: 96 Wells (12 x 8 Well strips)
  • BMPR1A Lyophilized Standard: 2
  • 100X Biotinylated BMPR1A Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: 0.057 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein Receptor 1A (BMPR1A). No significant cross-reactivity or interference between Bone Morphogenetic Protein Receptor 1A (BMPR1A) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Bmpr1a
• Gene Full Name: bone morphogenetic protein receptor, type 1A
• Alias Symbols: 1110037I22Rik;activin receptor-like kinase 3;AL;ALK3;ALK-3;AU045487;Bm;BMP;BMP type-1A receptor;BMP-2/BMP-4 receptor;Bmpr;BMPR-1A;BMPR-IA;bone morphogenetic protein receptor type-1A;serine/threonine-protein kinase receptor R5;SKR5.
• NCBI Gene Id: 12166
• Protein Name: P36895
• Description of Target: Bone morphogenetic protein receptor type-1A
• Uniprot ID: https://www.uniprot.org/uniprot/P36895
• Protein Accession #: NP_033888.2
• Nucleotide Accession #: NM_009758.4
• Protein Size (# AA): 532