Catalog No: OKEH00029
Size:96T
Price: $525.00
SKU
OKEH00029
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BMPR1A ELISA Kit (Human) : 96 Wells (OKEH00029)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology BMPR1A ELISA Kit (Human) (OKEH00029) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BMPR1A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BMPR1A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BMPR1A captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 108%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.6% (n = 20)
Mean Inter-assay CV%: < 9.9% (n = 20)
ELISA Kit Component
ComponentAmount
BMPR1A Microplate96 Wells (12 x 8 Well strips)
BMPR1A Lyophilized Standard2
100X Biotinylated BMPR1A Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.11 ng/mL
SpecificityNatural and recombinant Human Bone morphogenetic protein receptor type-1A
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolBMPR1A
Gene Full Namebone morphogenetic protein receptor type 1A
Alias Symbols10q23del;activin A receptor, type II-like kinase 3;activin receptor-like kinase 3;ACVRLK3;ALK3;ALK-3;BMP type-1A receptor;BMPR-1A;bone morphogenetic protein receptor type-1A;bone morphogenetic protein receptor, type IA;CD292;serine/threonine-protein kinase receptor R5;SKR5.
NCBI Gene Id657
Protein NameP36894
Description of TargetBone morphogenetic protein receptor type-1A
Uniprot IDhttps://www.uniprot.org/uniprot/P36894
Protein Accession #NP_004320
Nucleotide Accession #NM_004329
Protein Size (# AA)532
Molecular Weight60 kDa
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