BMP6 ELISA Kit (Rat) (OKCD02314)

Catalog No: OKCD02314

Size:96WELLS

Rat BMP6 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	92-103%	86-95%	99-105%	94-102%
  EDTA Plasma (n=5)	84-105%	91-105%	97-104%	88-97%
  Heparin Plasma (n=5)	86-101%	83-92%	84-101%	90-103%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein 6 (BMP6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein 6 (BMP6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bone Morphogenetic Protein 6 (BMP6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Bone Morphogenetic Protein 6 (BMP6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	78-96	81
  EDTA Plasma (n=5)	95-105	99
  Heparin Plasma(n=5)	82-99	95

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein 6 (BMP6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein 6 (BMP6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-BMP6 Microplate: 96 Wells (12 x 8 Well strips)
  • BMP6 Lyophilized Standard: 2
  • 100X Biotinylated BMP6 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 12.7 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein 6 (BMP6). No significant cross-reactivity or interference between Bone Morphogenetic Protein 6 (BMP6) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Bmp6
• Gene Full Name: bone morphogenetic protein 6
• Alias Symbols: BMP-6;bone morphogenetic protein 6;VG-1-related protein;VGR;VGR-1.
• NCBI Gene Id: 25644
• Protein Name: Bone morphogenetic protein 6
• Description of Target: Induces cartilage and bone formation.
• Uniprot ID: Q04906
• Protein Accession #: NP_037239.1
• Nucleotide Accession #: NM_013107.1
• Protein Size (# AA): 506